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Pepper blight disease inhibition metagenome clone screening using soil metagenome library [Korean Sources metagenome library Korean sources]

Title
Pepper blight disease inhibition metagenome clone screening using soil metagenome library [Korean Sources metagenome library Korean sources]
Authors
Park H.-C.Sung S.-R.Kim D.-G.Koo B.-S.Jeong B.Kim J.Yoon M.-Y.
Ewha Authors
정병문김진흥
SCOPUS Author ID
정병문scopus; 김진흥scopus
Issue Date
2009
Journal Title
Korean Journal of Microbiology
ISSN
0440-2413JCR Link
Citation
vol. 45, no. 2, pp. 228 - 231
Indexed
SCOPUS; KCI scopus
Abstract
We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease. © 2009, The Microbiological Society of Korea.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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