Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.contributor.author | 이수영 | * |
dc.contributor.author | 이화정 | * |
dc.contributor.author | 최선 | * |
dc.date.accessioned | 2016-08-29T12:08:10Z | - |
dc.date.available | 2016-08-29T12:08:10Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 2045-2322 | * |
dc.identifier.other | OAK-16651 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231162 | - |
dc.description.abstract | Osteoclast cells (OCs) are differentiated from bone marrow-derived macrophages (BMMs) by activation of receptor activator of nuclear factor kappa B (NF-kappa B) ligand (RANKL). Activation of NADPH oxidase (Nox) isozymes is involved in RANKL-dependent OC differentiation, implicating Nox isozymes as therapeutic targets for treatment of osteoporosis. Here, we show that a novel pyrazole derivative, Ewha-18278 has high inhibitory potency on Nox isozymes. Blocking the activity of Nox with Ewha-18278 inhibited the responses of BMMs to RANKL, including reactive oxygen species (ROS) generation, activation of mitogen-activated protein (MAP) kinases and NF-kappa B, and OC differentiation. To evaluate the anti-osteoporotic function of Ewha-18278, the derivative was applied to estrogen-deficient ovariectomized (OVX) ddY mice. Oral administration of Ewha-18278 (10 mg/kg/daily, 4 weeks) into the mice recovered bone mineral density, trabecular bone volume, trabecular bone length, number and thickness, compared to control OVX ddY mice. Moreover, treatment of OVX ddY mice with Ewha-18278 increased bone strength by increasing cortical bone thickness. We provide that Ewha-18278 displayed Nox inhibition and blocked the RANKL-dependent cell signaling cascade leading to reduced differentiation of OCs. Our results implicate Ewha-18278 as a novel therapeutic agent for the treatment of osteoporosis. | * |
dc.language | English | * |
dc.publisher | NATURE PUBLISHING GROUP | * |
dc.title | A novel pyrazole derivative protects from ovariectomy-induced osteoporosis through the inhibition of NADPH oxidase | * |
dc.type | Article | * |
dc.relation.volume | 6 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | SCIENTIFIC REPORTS | * |
dc.identifier.doi | 10.1038/srep22389 | * |
dc.identifier.wosid | WOS:000372028100001 | * |
dc.identifier.scopusid | 2-s2.0-84960947591 | * |
dc.author.google | Joo, Jung Hee | * |
dc.author.google | Huh, Jeong-Eun | * |
dc.author.google | Lee, Jee Hyun | * |
dc.author.google | Park, Doo Ri | * |
dc.author.google | Lee, Yoonji | * |
dc.author.google | Lee, Seul Gee | * |
dc.author.google | Choi, Sun | * |
dc.author.google | Lee, Hwa Jeong | * |
dc.author.google | Song, Seong-Won | * |
dc.author.google | Jeong, Yongmi | * |
dc.author.google | Goo, Ja-Il | * |
dc.author.google | Choi, Yongseok | * |
dc.author.google | Baek, Hye Kyung | * |
dc.author.google | Yi, Sun Shin | * |
dc.author.google | Park, Soo Jin | * |
dc.author.google | Lee, Ji Eun | * |
dc.author.google | Ku, Sae Kwang | * |
dc.author.google | Lee, Won Jae | * |
dc.author.google | Lee, Kee-In | * |
dc.author.google | Lee, Soo Young | * |
dc.author.google | Bae, Yun Soo | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.contributor.scopusid | 이수영(53980218900;7409697278) | * |
dc.contributor.scopusid | 이화정(57102029300) | * |
dc.contributor.scopusid | 최선(8659831000) | * |
dc.date.modifydate | 20240415133331 | * |