Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이대기 | * |
dc.date.accessioned | 2016-08-29T12:08:49Z | - |
dc.date.available | 2016-08-29T12:08:49Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 0013-7227 | * |
dc.identifier.issn | 1945-7170 | * |
dc.identifier.other | OAK-16274 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230973 | - |
dc.description.abstract | Mice carrying a targeted disruption of the prostaglandin E-2 (PGE(2)) E-prostanoid receptor 3 (EP3) gene, Ptger3, were fed a high-fat diet (HFD), or a micronutrient matched control diet, to investigate the effects of disrupted PGE(2)-EP3 signaling on diabetes in a setting of diet-induced obesity. Although no differences in body weight were seen in mice fed the control diet, when fed a HFD, EP3(-/-) mice gained more weight relative to EP3(+/+) mice. Overall, EP3(-/-) mice had increased epididymal fat mass and adipocyte size; paradoxically, a relative decrease in both epididymal fat pad mass and adipocyte size was observed in the heaviest EP3(-/-) mice. The EP3(-/-) mice had increased macrophage infiltration, TNF-alpha, monocyte chemoattractant protein-1, IL-6 expression, and necrosis in their epididymal fat pads as compared with EP3(+/+) animals. Adipocytes isolated from EP3(+/+) or EP3(-/-) mice were assayed for the effect of PGE(2)-evoked inhibition of lipolysis. Adipocytes isolated from EP3(-/-) mice lacked PGE(2)-evoked inhibition of isoproterenol stimulated lipolysis compared with EP3(+/+). EP3(-/-) mice fed HFD had exaggerated ectopic lipid accumulation in skeletal muscle and liver, with evidence of hepatic steatosis. Both blood glucose and plasma insulin levels were similar between genotypes on a control diet, but when fed HFD, EP3(-/-) mice became hyperglycemic and hyperinsulinemic when compared with EP3(+/+) fed HFD, demonstrating a more severe insulin resistance phenotype in EP3(-/-). These results demonstrate that when fed a HFD, EP3(-/-) mice have abnormal lipid distribution, developing excessive ectopic lipid accumulation and associated insulin resistance. | * |
dc.language | English | * |
dc.publisher | OXFORD UNIV PRESS INC | * |
dc.title | The PGE(2) EP3 Receptor Regulates Diet-Induced Adiposity in Male Mice | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 157 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 220 | * |
dc.relation.lastpage | 232 | * |
dc.relation.journaltitle | ENDOCRINOLOGY | * |
dc.identifier.doi | 10.1210/en.2015-1693 | * |
dc.identifier.wosid | WOS:000369962600026 | * |
dc.identifier.scopusid | 2-s2.0-84954526085 | * |
dc.author.google | Ceddia, Ryan P. | * |
dc.author.google | Lee, DaeKee | * |
dc.author.google | Maulis, Matthew F. | * |
dc.author.google | Carboneau, Bethany A. | * |
dc.author.google | Threadgill, David W. | * |
dc.author.google | Poffenberger, Greg | * |
dc.author.google | Milne, Ginger | * |
dc.author.google | Boyd, Kelli L. | * |
dc.author.google | Powers, Alvin C. | * |
dc.author.google | McGuinness, Owen P. | * |
dc.author.google | Gannon, Maureen | * |
dc.author.google | Breyer, Richard M. | * |
dc.contributor.scopusid | 이대기(37047040400) | * |
dc.date.modifydate | 20231120165418 | * |