Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2016-08-29T12:08:49Z | - |
dc.date.available | 2016-08-29T12:08:49Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.other | OAK-16259 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230969 | - |
dc.description.abstract | The transmembrane domain (TMD) of the syndecans, a family of transmembrane heparin sulfate proteoglycans, is involved in forming homo-and heterodimers and oligomers that transmit signaling events. Recently, we reported that the unique phenylalanine in TMD positively regulates intramolecular interactions of syndecan-2. Besides the unique phenylalanine, syndecan-2 contains a conserved phenylalanine (SDC2-Phe-169) that is present in all syndecan TMDs, but its function has not been determined. We therefore investigated the structural role of SDC2-Phe-169 in syndecan TMDs. Replacement of SDC2-Phe-169 by tyrosine (S2F169Y) did not affect SDS-resistant homodimer formation but significantly reduced SDS-resistant heterodimer formation between syndecan-2 and -4, suggesting that SDC2-Phe-169 is involved in the heterodimerization/oligomerization of syndecans. Similarly, in an in vitro binding assay, a syndecan-2 mutant (S2(F169Y)) showed a significantly reduced interaction with syndecan-4. FRET assays showed that heteromolecular interactions between syndecan-2 and -4 were reduced in HEK293T cells transfected with S2(F169Y) compared with syndecan-2. Moreover, S2(F169Y) reduced downstream reactions mediated by the heterodimerization of syndecan-2 and -4, including Rac activity, cell migration, membrane localization of PKC alpha, and focal adhesion formation. The conserved phenylalanine in syndecan-1 and -3 also showed heterodimeric interaction with syndecan-2 and -4. Taken together, these findings suggest that the conserved phenylalanine in the TMD of syndecans is crucial in regulating heteromeric interactions of syndecans. | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | The Conserved Phenylalanine in the Transmembrane Domain Enhances Heteromeric Interactions of Syndecans | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 291 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 872 | - |
dc.relation.lastpage | 881 | - |
dc.relation.journaltitle | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.identifier.doi | 10.1074/jbc.M115.685040 | - |
dc.identifier.wosid | WOS:000367830500033 | - |
dc.identifier.scopusid | 2-s2.0-84954194687 | - |
dc.author.google | Kwon, Mi-Jung | - |
dc.author.google | Park, Jisu | - |
dc.author.google | Jang, Sinae | - |
dc.author.google | Eom, Chi-Yong | - |
dc.author.google | Oh, Eok-Soo | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20230201093717 | - |