Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.contributor.author | 김재상 | * |
dc.date.accessioned | 2016-08-29T12:08:49Z | - |
dc.date.available | 2016-08-29T12:08:49Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1083-351X | * |
dc.identifier.other | OAK-16258 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230968 | - |
dc.description.abstract | We have previously reported that Ahnak-mediated TGF beta signaling leads to down-regulation of c-Myc expression. Here, we show that inhibition of Ahnak can promote generation of induced pluripotent stem cells (iPSC) via up-regulation of endogenous c-Myc. Consistent with the c-Myc inhibitory role of Ahnak, mouse embryonic fibroblasts from Ahnak-deficient mouse (Ahnak(-/-) MEF) show an increased level of c-Myc expression compared with wild type MEF. Generation of iPSC with just three of the four Yamanaka factors, Oct4, Sox2, and Klf4 (hereafter 3F), was significantly enhanced in Ahnak(-/-) MEF. Similar results were obtained when Ahnak-specific shRNA was applied to wild type MEF. Of note, expression of Ahnak was significantly induced during the formation of embryoid bodies from embryonic stem cells, suggesting that Ahnak-mediated c-Myc inhibition is involved in embryoid body formation and the initial differentiation of pluripotent stem cells. The iPSC from 3F-infected Ahnak(-/-) MEF cells (Ahnak(-/-) - iPSC-3F) showed expression of all stem cell markers examined and the capability to form three primary germ layers. Moreover, injection of Ahnak(-/-) - iPSC-3F into athymic nude mice led to development of teratoma containing tissues from all three primary germ layers, indicating that iPSC from Ahnak(-/-) MEF are bona fide pluripotent stem cells. Taken together, these data provide evidence for a new role for Ahnak in cell fate determination during development and suggest that manipulation of Ahnak and the associated signaling pathway may provide a means to regulate iPSC generation. | * |
dc.language | English | * |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | * |
dc.title | Regulation of c-Myc Expression by Ahnak Promotes Induced Pluripotent Stem Cell Generation | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 291 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 752 | * |
dc.relation.lastpage | 761 | * |
dc.relation.journaltitle | JOURNAL OF BIOLOGICAL CHEMISTRY | * |
dc.identifier.doi | 10.1074/jbc.M115.659276 | * |
dc.identifier.wosid | WOS:000367830500023 | * |
dc.identifier.scopusid | 2-s2.0-84954163900 | * |
dc.author.google | Lim, Hee Jung | * |
dc.author.google | Kim, Jusong | * |
dc.author.google | Park, Chang-Hwan | * |
dc.author.google | Lee, Sang A. | * |
dc.author.google | Lee, Man Ryul | * |
dc.author.google | Kim, Kye-Seong | * |
dc.author.google | Kim, Jaesang | * |
dc.author.google | Bae, Yun Soo | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.contributor.scopusid | 김재상(8643335800) | * |
dc.date.modifydate | 20240415133331 | * |