Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신윤용 | * |
dc.contributor.author | 김대기 | * |
dc.contributor.author | 임우성 | * |
dc.date.accessioned | 2016-08-29T12:08:41Z | - |
dc.date.available | 2016-08-29T12:08:41Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 1949-2553 | * |
dc.identifier.other | OAK-16004 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230893 | - |
dc.description.abstract | Distant relapse after chemotherapy is an important clinical issue for treating breast cancer patients and results from the development of cancer stem-like cells (CSCs) during chemotherapy. Here we report that blocking epithelial-to-mesenchymal transition (EMT) suppresses paclitaxel-induced CSCs properties by using a MDA-MB-231-xenografted mice model (in vivo), and breast cancer cell lines (in vitro). Paclitaxel, one of the cytotoxic taxane-drugs such as docetaxel, increases mesenchymal markers (Vimentin and Fibronectin) and decreases an epithelial marker (Zo-1). Blocking TGF-β signaling with the TGF-β type I receptor kinase (ALK5) inhibitor, EW-7197, suppresses paclitaxel-induced EMT and CSC properties such as mammosphere-forming efficiency (MSFE), aldehyde dehydrogenase (ALDH) activity, CD44+/CD24- ratio, and pluripotency regulators (Oct4, Nanog, Klf4, Myc, and Sox2). The combinatorial treatment of EW-7197 improves the therapeutic effect of paclitaxel by decreasing the lung metastasis and increasing the survival time in vivo. We confirmed that Snail is increased by paclitaxel-induced intracellular reactive oxygen species (ROS) and EW-7197 suppresses the paclitaxel-induced Snail and EMT by attenuating paclitaxel-induced intracellular ROS. Knock-down of SNAI1 suppresses paclitaxel-induced EMT and CSC properties. These data together suggest that blocking the Snail-induced EMT with the ALK5 inhibitor attenuates metastasis after paclitaxel-therapy and that this combinatorial approach could prove useful in treating breast cancer. | * |
dc.language | English | * |
dc.publisher | Impact Journals LLC | * |
dc.subject | Epithelial-to-mesenchymal transition (EMT) | * |
dc.subject | Metastasis | * |
dc.subject | Paclitaxel | * |
dc.subject | Snail | * |
dc.subject | Transforming growth factor-β (TGF-β) | * |
dc.title | Combinatorial TGF-β attenuation with paclitaxel inhibits the epithelial-to-mesenchymal transition and breast cancer stem-like cells | * |
dc.type | Article | * |
dc.relation.issue | 35 | * |
dc.relation.volume | 6 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 37526 | * |
dc.relation.lastpage | 37543 | * |
dc.relation.journaltitle | Oncotarget | * |
dc.identifier.doi | 10.18632/oncotarget.6063 | * |
dc.identifier.scopusid | 2-s2.0-84947733386 | * |
dc.author.google | Park S.-Y. | * |
dc.author.google | Kim M.-J. | * |
dc.author.google | Park S.-A. | * |
dc.author.google | Kim J.-S. | * |
dc.author.google | Min K.-N. | * |
dc.author.google | Kim D.-K. | * |
dc.author.google | Lim W. | * |
dc.author.google | Nam J.-S. | * |
dc.author.google | Sheen Y.Y. | * |
dc.contributor.scopusid | 신윤용(6603872711) | * |
dc.contributor.scopusid | 김대기(35083694200) | * |
dc.contributor.scopusid | 임우성(27167744500) | * |
dc.date.modifydate | 20240130112620 | * |