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Microsphere-Incorporated Hybrid Thermogel for Neuronal Differentiation of Tonsil Derived Mesenchymal Stem Cells

Title
Microsphere-Incorporated Hybrid Thermogel for Neuronal Differentiation of Tonsil Derived Mesenchymal Stem Cells
Authors
Patel M.Moon H.J.Jung B.K.Jeong B.
Ewha Authors
정병문
SCOPUS Author ID
정병문scopus
Issue Date
2015
Journal Title
Advanced Healthcare Materials
ISSN
2192-2640JCR Link
Citation
vol. 4, no. 10, pp. 1565 - 1574
Keywords
3D cultureDrug deliveryNeuronal differentiationSol-gel transitionStem cells
Publisher
Wiley-VCH Verlag
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Neuronal differentiation of tonsil-derived mesenchymal stem cells (TMSCs) is investigated in a 3D hybrid system. The hybrid system is prepared by increasing the temperature of poly(ethylene glycol)-poly(l-alanine) aqueous solution to 37 °C through the heat-induced sol-to-gel transition, in which TMSCs and growth factor releasing microspheres are suspended. The in situ formed gel exhibits a modulus of 800 Pa at 37 °C, similar to that of brain tissue, and it is robust enough to hold the microspheres and cells during the 3D culture of TMSCs. The neuronal growth factors are released over 12-18 d, and the TMSCs in a spherical shape initially undergo multipolar elongation during the 3D culture. Significantly higher expressions of the neuronal biomarkers such as nuclear receptor related protein (Nurr-1), neuron specific enolase, microtubule associated protein-2, neurofilament-M, and glial fibrillary acidic protein are observed in both mRNA level and protein level in the hybrid systems than in the control experiments. This study proves the significance of a controlled drug delivery concept in tissue engineering or regenerative medicine, and a 3D hybrid system with controlled release of growth factors from microspheres in a thermogel can be a very promising tool. A microsphere-incorporated hybrid hydrogel is prepared by thermogellation of a microsphere- and stem cell-suspended poly(ethylene glycol)-b-poly(l-alanine) aqueous solution. The controlled supply of neuronal growth factors from the microspheres to the coencapsulated tonsil derived mesenchymal stem cells provides an effective tool in controlling neuronal differentiation of the stem cells. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/adhm.201500224
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자연과학대학 > 화학·나노과학전공 > Journal papers
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