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A preliminary X-ray study of 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei

Title
A preliminary X-ray study of 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei
Authors
Park J.Lee D.Kim M.-S.Kim D.Y.Shin D.H.
Ewha Authors
신동해
SCOPUS Author ID
신동해scopus
Issue Date
2015
Journal Title
Acta Crystallographica Section F:Structural Biology Communications
ISSN
2053230XJCR Link
Citation
vol. 71, pp. 790 - 793
Keywords
3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphataseBurkholderia pseudomalleiYrbI
Publisher
International Union of Crystallography
Indexed
SCIE; SCOPUS WOS scopus
Abstract
3-Deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI), the third enzyme in the pathway for the biosynthesis of 3-deoxy-d-manno-oct-2-ulosonic acid (KDO), hydrolyzes KDO 8-phosphate to KDO and inorganic phosphate. YrbI belongs to the haloacid dehalogenase (HAD) superfamily, which is a large family of magnesium-dependent phosphatase/phospho transferase enzymes. In this study, YrbI from Burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.25 Å resolution. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 63.7, b = 97.5, c = 98.0 Å. A full structural determination is in progress to elucidate the structure-function relationship of this protein. © 2015 International Union of Crystallography.
DOI
10.1107/S2053230X15006135
Appears in Collections:
약학대학 > 약학과 > Journal papers
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