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The effect of umbilical cord blood derived mesenchymal stem cells in monocrotaline-induced pulmonary artery hypertension rats

Title
The effect of umbilical cord blood derived mesenchymal stem cells in monocrotaline-induced pulmonary artery hypertension rats
Authors
Lee H.Lee J.C.Kwon J.H.Kim K.C.Cho M.-S.Yang Y.S.Oh W.Choi S.J.Seo E.-S.Lee S.-J.Wang T.J.Hong Y.M.
Ewha Authors
홍영미조민선김관창권정현
SCOPUS Author ID
홍영미scopus; 조민선scopus; 김관창scopus
Issue Date
2015
Journal Title
Journal of Korean Medical Science
ISSN
1011-8934JCR Link
Citation
vol. 30, no. 5, pp. 576 - 585
Keywords
Cord blood stem cell transfusionGene ExpressionHypertensionMonocrotalinePulmonary
Publisher
Korean Academy of Medical Science
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)- induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH. © 2015 The Korean Academy of Medical Sciences.
DOI
10.3346/jkms.2015.30.5.576
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의과대학 > 의학과 > Journal papers
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