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Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis

Title
Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
Authors
Kim T.G.Jeong S.-Y.Cho K.-S.
Ewha Authors
조경숙김태관
SCOPUS Author ID
조경숙scopus
Issue Date
2014
Journal Title
Biotechnology Reports
ISSN
2215-017XJCR Link
Citation
vol. 4, no. 1, pp. 1 - 4
Keywords
Anaerobic digesterDroplet-digital PCRmcrAMethanogen communityQuantitative real-time PCR
Publisher
Elsevier
Indexed
SCOPUS scopus
Abstract
Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copiesmL-DNA extract-1). The mcrA-based methanogen communities in three full-scale anaerobic digesters were examined using the two technologies. dd-PCR detected seven groups from the digesters, while qPCR did five groups, indicating that dd-PCR is more sensitive for DNA quantification. Linear regression showed quantitative agreements between both of the technologies (R2 = 0.59-0.98) in the five groups that were concurrently detected. Principal component analysis from the two datasets consistently indicated a substantial difference in the community composition among the digesters and revealed similar levels of differentiation among the communities. The combined results suggest that dd-PCR is more promising for examining methanogenic archaeal communities in biotechnological processes. © 2014 The Authors. Published by Elsevier B.V.
DOI
10.1016/j.btre.2014.06.010
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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