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Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst

Title
Biotransformation of oleic acid into 10-ketostearic acid by recombinant Corynebacterium glutamicum-based biocatalyst
Authors
Lee B.Lee S.Kim H.Jeong K.Park J.Lee E.Lee J.
Ewha Authors
박진병
SCOPUS Author ID
박진병scopus
Issue Date
2015
Journal Title
Biotechnology Letters
ISSN
1415-5492JCR Link
Citation
vol. 37, no. 5, pp. 1101 - 1106
Keywords
10-Ketostearic acidBiocatalystsBiotransformationCorynebacterium glutamicumOleate hydrataseOleic acidSecondary alcohol dehydrogenase
Publisher
Kluwer Academic Publishers
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Objective: To produce 10-ketostearic acid from oleic acid.Conclusion: This is the first report of 10-ketostearic acid production using a recombinant C. glutamicum.Results: Oleic acid was converted to 10-ketostearic acid by a recombinant Corynebacterium glutamicum ATCC 13032 expressing oleate hydratase from Stenotrophomonas maltophilia and a secondary alcohol dehydrogenase from Micrococcus luteus under the control of a synthetic constitutive promoter. Optimal conditions for 10-ketostearic acid production were pH 7.5 and 30 °C with 5 g cells l−1 and 2.5 g oleic acid l−1. Under these conditions, the cells produced 1.96 g 10-ketostearic acid l−1 from oleic acid in 6 h, with a conversion yield of 78 % (w) and a maximum volumetric productivity of 1.67 g l−1 h−1. © 2015, Springer Science+Business Media Dordrecht.
DOI
10.1007/s10529-015-1775-7
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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