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Regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (YeaT)

Title
Regulation of aerobic and anaerobic D-malate metabolism of Escherichia coli by the LysR-type regulator DmlR (YeaT)
Authors
Lukas H.Reimann J.Kim O.B.Grimpo J.Unden G.
Ewha Authors
김옥빈
SCOPUS Author ID
김옥빈scopus
Issue Date
2010
Journal Title
Journal of Bacteriology
ISSN
0021-9193JCR Link
Citation
vol. 192, no. 10, pp. 2503 - 2511
Indexed
SCI; SCIE; SCOPUS scopus
Abstract
Escherichia coli K-12 is able to grow under aerobic conditions on D-malate using DctA for D-malate uptake and the D-malate dehydrogenase DmlA (formerly YeaU) for converting D-malate to pyruvate. Induction of dmlA encoding DmlA required an intact dmlR (formerly yeaT) gene, which encodes DmlR, a LysR-type transcriptional regulator. Induction of dmlA by DmlR required the presence of D-malate or L- or meso-tartrate, but only D-malate supported aerobic growth. The regulator of general C 4-dicarboxylate metabolism (DcuS-DcuR two-component system) had some effect on dmlA expression. The anaerobic L-tartrate regulator TtdR or the oxygen sensors ArcB-ArcA and FNR did not have a major effect on dmlA expression. DmlR has a high level of sequence identity (49%) with TtdR, the L- and meso-tartrate-specific regulator of L-tartrate fermentation in E. coli. dmlA was also expressed at high levels under anaerobic conditions, and the bacteria had D-malate dehydrogenase activity. These bacteria, however, were not able to grow on D-malate since the anaerobic pathway for D-malate degradation has a predicted yield of ≤0 ATP/mol D-malate. Slow anaerobic growth on D-malate was observed when glycerol was also provided as an electron donor, and D-malate was used in fumarate respiration. The expression of dmlR is subject to negative autoregulation. The network for regulation and coordination of the central and peripheral pathways for C 4- dicarboxylate metabolism by the regulators DcuS-DcuR, DmlR, and TtdR is discussed. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
DOI
10.1128/JB.01665-09
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자연과학대학 > 생명과학전공 > Journal papers
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