Characterization of cyclohexane and hexane degradation by Rhodococcus SP. EC1

Abstract

Cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. In this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. Based on a 16S rDNA PCR-DGGE (denaturing gradient gel electrophoresis) method, this consortium was identified as comprising alpha-proteobacteria, actionbacteria, and gamma-proteobacteria. One of these organisms, Rhodococcus sp. EC1, was isolated and shown to have excellent cyclohexane-degrading ability. The maximum specific cyclohexane degradation rate (V max) for EC1 was 246.4 umolg-DCW -1 (dry cell weight) -h -1. In addition to its cyclohexane degradation abilities, EC1 was also able to strongly degrade hexane, with a maximum specific hexane degradation rate of 361 μmol·g-DCW -1·h -1. Experiments using 14C-hexane revealed that EC1 mineralized 40.3% of hexane into CO 2and converted 52.8% into biomass. Moreover, EC1 could use other hydrocarbons, including methanol, ethanol, acetone, methyl tert-butyl ether (MTBE), pyrene, diesel, lubricant oil, benzene, toluene, ethylbenzene, m-xylene, p-xylene and oxylene. These findings collectively suggest that EC1 may be a useful biological resource for removal of cyclohexane, hexane, and other recalcitrant hydrocarbons.