Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오구택 | * |
dc.date.accessioned | 2016-08-28T11:08:52Z | - |
dc.date.available | 2016-08-28T11:08:52Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 1582-1838 | * |
dc.identifier.other | OAK-13149 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/229181 | - |
dc.description.abstract | Imaging or drug delivery tools for atherosclerosis based on the plaque biology are still insufficient. Here, we attempted to identify peptides that selectively home to atherosclerotic plaques using phage display. A phage library containing random peptides was ex viv screened for binding to human atheroma tissues. After three to four rounds of selection, the DNA inserts of phage clones wer sequenced. A peptide sequence, CRKRLDRNC, was the most frequently occurring one. Intravenously injected phage displaying the CRKRLDRNC peptide was observed to home to atherosclerotic aortic tissues of low-density lipoprotein receptor-deficient (Ldlr-/-) mice at higher levels than to normal aortic tissues of wild-type mice. Moreover, a fluorescein- or radioisotope-conjugated synthetic CRKRLDRNC peptide, but not a control peptide, homed in vivo to atherosclerotic plaques in Ldlr-/- mice, while homing of the peptide to other organs such as brain was minimal. The homing peptide co-localized with endothelial cells, macrophages and smooth muscle cells a mouse and human atherosclerotic plaques. Homology search revealed that the CRKRLDRNC peptide shares a motif of interleukin-receptor (IL-4) that is critical for binding to its receptor. The peptide indeed co-localized with IL-4 receptor (IL-4R) at atherosclerotic plaques. Moreover, the peptide bound to cultured cells expressing IL-4R on the cell surface and the binding was inhibited by the knock-down of IL-4R. These results show that the CRKRLDRNC peptide homes to atherosclerotic plaques through binding to IL-4R as its target and may be a useful tool for selective drug delivery and molecular imaging of atherosclerosis. © 2007 The Authors. | * |
dc.language | English | * |
dc.title | Phage display selection of peptides that home to atherosclerotic plaques: IL-4 receptor as a candidate target in atherosclerosis | * |
dc.type | Article | * |
dc.relation.issue | 5B | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2003 | * |
dc.relation.lastpage | 2014 | * |
dc.relation.journaltitle | Journal of Cellular and Molecular Medicine | * |
dc.identifier.doi | 10.1111/j.1582-4934.2008.00189.x | * |
dc.identifier.wosid | WOS:000260538300018 | * |
dc.identifier.scopusid | 2-s2.0-55149086977 | * |
dc.author.google | Hong H.-Y. | * |
dc.author.google | Lee H.Y. | * |
dc.author.google | Kwak W. | * |
dc.author.google | Yoo J. | * |
dc.author.google | Na M.-H. | * |
dc.author.google | So I.S. | * |
dc.author.google | Kwon T.-H. | * |
dc.author.google | Park H.-S. | * |
dc.author.google | Huh S. | * |
dc.author.google | Oh G.T. | * |
dc.author.google | Kwon I.-C. | * |
dc.author.google | Kim I.-S. | * |
dc.author.google | Lee B.-H. | * |
dc.contributor.scopusid | 오구택(7007056663) | * |
dc.date.modifydate | 20240123094756 | * |