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Characterization of DNA-dye complexes on nanostructure arrays

Title
Characterization of DNA-dye complexes on nanostructure arrays
Authors
Kim S.Kim Y.S.Lee M.
Ewha Authors
이민영
SCOPUS Author ID
이민영scopus
Issue Date
2006
Journal Title
Current Applied Physics
ISSN
1567-1739JCR Link
Citation
vol. 6, no. SUPPL. 1, pp. e251 - e256
Indexed
SCI; SCIE; SCOPUS; KCI scopus
Abstract
The binding of dimethyldiazaperopyrenium dication with Calf Thymus DNA has been studied by atomic force and optical microscopy. We obtained bulk and single dye-intercalating DNA's fluorescence lifetime decay curves and lifetime distributions. We placed the DNA-dye complexes into different sizes of nanostructures which were prepared on cover glasses using PDMS and UV-curing polyurethane materials. The correlation with dye-intercalating DNA complexes and patterns were observed by acquiring AFM images of nano- and micropatterned cover glasses and DNA-dye complexes. According to the size of channel width, DNA-dye complexes show multiformity. We acquired lifetime images and distributions of dye-intercalating DNA complexes coated on cover glasses using time-correlated confocal fluorescence microscope apparatus. We also obtained information of surface topology and 2D images of fluorescence lifetime at the same area in a continuous series. © 2006 Elsevier B.V. All rights reserved.
DOI
10.1016/j.cap.2006.01.050
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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