Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배윤수 | * |
dc.date.accessioned | 2016-08-28T11:08:30Z | - |
dc.date.available | 2016-08-28T11:08:30Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-12857 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/228922 | - |
dc.description.abstract | Reactive oxygen species, such as the superoxide anion, H2O 2, and the hydroxyl radical, have been considered as cytotoxic by-products of cellular metabolism. However, recent studies have provided evidence that H2O2 serves as a signaling molecule modulating various physiological functions. Here we investigated the effect of H2O2 on the regulation of intracellular Ca2+ signaling in rat cortical astrocytes. H2O2 triggered the generation of oscillations of intracellular Ca2+ concentration ([Ca2+]i) in a concentration-dependent manner over the range 10-100 μM. The H2O2-induced [Ca 2+]i oscillations persisted in the absence of extracellular Ca2+ and were prevented by depletion of intracellular Ca2+ stores with thapsigargin. The H2O2-induced [Ca2+]i oscillations were not inhibited by pretreatment with ryanodine but were prevented by 2-aminoethoxydiphenyl borate and caffeine, known antagonists of inositol 1,4,5-trisphosphate receptors. H2O 2 activated phospholipase C (PLC) γ1 in a dose-dependent manner, and U73122, an inhibitor of PLC, completely abolished the H 2O2-induced [Ca2+]i oscillations. In addition, RNA interference against PLCγ1 and the expression of the inositol 1,4,5-trisphosphate-sequestering "sponge" prevented the generation of [Ca2+]i oscillations. H2O 2-induced [Ca2+]i oscillations and PLCγ1 phosphorylation were inhibited by pretreatment with dithiothreitol, a sulfhydryl-reducing agent. Finally, epidermal growth factor induced H 2O2 production, PLCγ1 activation, and [Ca 2+]i increases, which were attenuated by N-acetylcysteine and diphenyleneiodonium and by the overexpression of peroxiredoxin type II. Therefore, we conclude that low concentrations of exogenously applied H 2O2 generate [Ca2+]i oscillations by activating PLCγ1 through sulfhydryl oxidation-dependent mechanisms. Furthermore, we show that this mechanism underlies the modulatory effect of endogenously produced H2O2 on epidermal growth factor-induced Ca2+ signaling in rat cortical astrocytes. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc. | * |
dc.language | English | * |
dc.title | Critical role of phospholipase Cγ1 in the generation of H 2O2-evoked [Ca2+]i oscillations in cultured rat cortical astrocytes | * |
dc.type | Article | * |
dc.relation.issue | 19 | * |
dc.relation.volume | 281 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 13057 | * |
dc.relation.lastpage | 13067 | * |
dc.relation.journaltitle | Journal of Biological Chemistry | * |
dc.identifier.doi | 10.1074/jbc.M601726200 | * |
dc.identifier.wosid | WOS:000237336600009 | * |
dc.identifier.scopusid | 2-s2.0-33744948517 | * |
dc.author.google | Jeong H.H. | * |
dc.author.google | Seok J.M. | * |
dc.author.google | Hae M.B. | * |
dc.author.google | Min S.K. | * |
dc.author.google | Jo H. | * |
dc.author.google | Yun S.B. | * |
dc.author.google | Lee S.-I. | * |
dc.author.google | Bootman M.D. | * |
dc.author.google | Roderick H.L. | * |
dc.author.google | Dong M.S. | * |
dc.author.google | Jeong T.S. | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.date.modifydate | 20240415133331 | * |