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Study on the molecular weights of radioprotective ginseng proteins by HPLC method

Title
Study on the molecular weights of radioprotective ginseng proteins by HPLC method
Authors
Kim C.Kim H.L.
Ewha Authors
김춘미
SCOPUS Author ID
김춘미scopus
Issue Date
1986
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
vol. 9, no. 1, pp. 5 - 9
Publisher
Pharmaceutical Society of Korea
Indexed
SCIE; SCOPUS; KCI scopus
Abstract
Partially purified ginseng proteins were either treated with sodium dodecyl sulfate (SDS) and β-mercaptoethanol to denature the proteins or not, and subjected to Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) to compare the components of each fraction. Standard proteins of known molecular weights (MW) were also either treated with SDS and β-mercaptoethanol or not, and subjected to HPLC to obtain regression lines for MW determination. From the retention times obtained from samples in either case by HPLC, the MW were estimated as following. In SDS treated condition, GI fraction showed three peaks each with MW of above 100,000, 51,000 and 19,000. GII showed one original peak with MW of 21,000 and GIII, two peaks each with MW of 19,000 and 14,000. On the other hand, in non-SDS treated condition, GI fraction showed two peaks each with MW of above 200,000 and 52,000. GII showed one original peak with MW of 41,000 and GIII, three peaks each with MW of 28,000, 19,000 and 14,000. © 1986 The Pharmaceutical Society of Korea.
DOI
10.1007/BF02857698
Appears in Collections:
약학대학 > 약학과 > Journal papers
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