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Trichostatin A attenuates airway inflammation in mouse asthma model

Title
Trichostatin A attenuates airway inflammation in mouse asthma model
Authors
Choi J.-H.Oh S.-W.Kang M.-S.Kwon H.J.Oh G.-T.Kim D.-Y.
Ewha Authors
오구택
SCOPUS Author ID
오구택scopus
Issue Date
2005
Journal Title
Clinical and Experimental Allergy
ISSN
0954-7894JCR Link
Citation
vol. 35, no. 1, pp. 89 - 96
Indexed
SCI; SCIE; SCOPUS scopus
Abstract
Background: Histone deacetylase (HDAC) inhibition has been demonstrated to change the expression of a restricted set of cellular genes. T cells are essential in the pathogenesis of allergen-induced airway inflammation. It was recently reported that treatment with HDAC inhibitors induces a T cell-suppressive effect. Objective: The purpose of this study was to determine whether treatment with trichostatin A (TSA), a representative HDAC inhibitor, would reduce allergen-induced airway inflammation in a mouse asthma model. Methods: BALB/c mice were intraperitoneally sensitized to ovalbumin (OVA) and challenged with an aerosol of OVA. TSA (1 mg/kg body weight) was injected intraperitoneally every 2 days beginning on day 1. Mouse lungs were assayed immunohistochemically for HDACl, a major HDAC subtype, and for infiltration of CD4+ cells. The effect of TSA on airway hyper-responsiveness (AHR) was determined, and the bronchoalveolar lavage fluid (BALF) of these mice was assayed for the number and types of inflammatory cells, and for the concentrations of IL-4, IL-5, and IgE. Results HDACl was localized within most airway cells and infiltrating inflammatory cells of asthmatic lungs. Treatment with TSA significantly attenuated AHR, as well as the numbers of eosinophils and lymphocytes in BALF. TSA also reduced infiltration of CD4+ and inflammatory cells and mucus occlusions in lung tissue, and decreased the concentrations of IL-4, IL-5, and IgE in BALF. Conclusion: TSA attenuated the development of allergic airway inflammation by decreasing expression of the Th2 cytokines, IL-4 and IL-5, and IgE, which resulted from reduced T cell infiltration. Our results suggest that HDAC inhibition may attenuate the development of asthma by a T cell suppressive effect.
DOI
10.1111/j.1365-2222.2004.02006.x
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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