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Homeodomain protein CDX2 regulates COX-2 expression in colorectal cancer

Title
Homeodomain protein CDX2 regulates COX-2 expression in colorectal cancer
Authors
Kim S.-P.Park J.-W.Lee S.-H.Lim J.H.Jang B.-C.Jang I.-H.Freund J.-N.Suh S.-I.Mun K.C.Song D.-K.Ha E.-M.Lee W.-J.Kwon T.K.
Ewha Authors
이원재
Issue Date
2004
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 315, no. 1, pp. 93 - 99
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
CDX2 is an intestine-specific tumor suppressor gene encoding homeodomain-containing transcription factor, which is involved in a variety of developmental, proliferating, and differentiating processes. Moreover, the expression of CDX2 is reduced in a subset of primary colorectal cancers. In contrast, cyclooxygenase-2 (COX-2) is often up-regulated in human colorectal cancers. However, the molecular relationship between CDX2 down-regulation and COX-2 up-regulation is unknown. Here we show that CDX2 down-regulates COX-2 promoter activity by interacting with NF-κB. The ectopic expression of CDX2 was found to suppress PMA-induced COX-2 promoter activity in a dose-dependent manner. In addition, the treatment of colorectal cancer cells with PMA resulted in significant reduction in the level of endogenous CDX2 and a significant increase in the level of endogenous COX-2, in a dose-dependent manner. Furthermore, CDX2 was found to co-immunoprecipitate with the p65 subunit of NF-κB and to inhibit p65-induced NF-κB minimal promoter activity in colon cancer cells. These results suggest that reduced CDX2 expression may be involved in colorectal carcinogenesis by enhancing NF-κB-mediated inflammatory genes such as COX-2. © 2004 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2004.01.020
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일반대학원 > 바이오융합과학과 > Journal papers
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