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Optimization of Subcritical Water Extraction of Flavanols from Green Tea Leaves
- Optimization of Subcritical Water Extraction of Flavanols from Green Tea Leaves
- Ko, Min-Jung; Cheigh, Chan-Ick; Chung, Myong-Soo
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- 0021-8561; 1520-5118
- vol. 62, no. 28, pp. 6828 - 6833
- subcritical-water extraction; flavanols; epimerization; green tea leaves
- AMER CHEMICAL SOC
- SCI; SCIE; SCOPUS
- The subcritical-water extraction (SWE) of six kinds of flavanols from green tea leaves and the effect of extraction conditions were investigated by varying the temperature and time. The maximum yield of total flavanols, 71.36 +/- 4.23 mg/g green tea leaves (mean +/- SD), was obtained under extraction temperature/time conditions of 150 degrees C/5 min. The efficiency of SWE for total flavanols was slightly higher than that of the conventional extraction solvents such as methanol and ethanol. The extraction of flavanols via SWE was specifically adequate for epimer structures such as catechin, catechin gallate, and gallocatechin gallate due to the epimerization of epicatechins. The extraction efficiency of epirners was increased at temperatures up to 170 degrees C, whereas that of epicatechins was decreased. Thus, most epicatechins were converted to epimers during SWE, leading to some flavanol destruction at high temperatures, except when a short extraction time of 5 min was used.
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