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Real-time monitoring of adipocyte differentiation using a capacitance sensor array

Title
Real-time monitoring of adipocyte differentiation using a capacitance sensor array
Authors
Lee R.Jung I.Park M.Ha H.Yoo K.H.
Ewha Authors
하헌주
SCOPUS Author ID
하헌주scopus
Issue Date
2013
Journal Title
Lab on a Chip - Miniaturisation for Chemistry and Biology
ISSN
1473-0197JCR Link
Citation
vol. 13, no. 17, pp. 3410 - 3416
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
As obesity and its associated metabolic diseases become a worldwide epidemic, the demand for novel anti-obesity agents is increasing. We report a label-free and real-time monitoring method that uses a capacitance sensor array to screen anti-obesity agents. The results for the real-time capacitance of 3T3-L1 cells treated with 12 different chemicals extracted from natural products were consistent with the biochemical indicators of adipogenesis such as the expression of perilipin, the major protein coating the surface of lipid droplets in adipocytes. The data demonstrate that a capacitance change during adipocyte differentiation is closely associated with lipid accumulation in the cells, suggesting that adipocyte differentiation can be monitored in real time. This capacitance sensor might be used for label-free and real-time monitoring of adipocyte differentiation, and may facilitate the development of high throughput screening methods for anti-obesity drugs. This journal is © The Royal Society of Chemistry 2013.
DOI
10.1039/c3lc50453k
Appears in Collections:
약학대학 > 약학과 > Journal papers
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