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A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced hocl production

Title
A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced hocl production
Authors
Xu Q.Lee K.-A.Lee S.Lee K.M.Lee W.-J.Yoon J.
Ewha Authors
윤주영Qingling Xu
SCOPUS Author ID
윤주영scopus
Issue Date
2013
Journal Title
Journal of the American Chemical Society
ISSN
0002-7863JCR Link
Citation
vol. 135, no. 26, pp. 9944 - 9949
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Oxidative stress induced by reactive oxygen species (ROS) plays crucial roles in a wide range of physiological processes and is also implicated in various diseases, including cancer, chronic inflammatory diseases, and neurodegenerative disorders. Among the various ROS, hypochlorous acid (HOCl) plays as a powerful microbicidal agent in the innate immune system. The regulated production of microbicidal HOCl is required for the host to control the invading microbes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image ROS. However, it is still challenging to design a fluorescent probe with exclusive selectivity toward a particular member of ROS. In the current work, we designed FBS as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad range of pH. Compared with other sensors, the "dual-lock" structure of FBS has an advantage of eliminating interferences from other ROS/RNS. Importantly, we further showed that our HOCl probe could be applied for the in vivo imaging of physiological HOCl production in the mucosa of live animals. This probe provides a promising tool for the study of HOCl production. © 2013 American Chemical Society.
DOI
10.1021/ja404649m
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자연과학대학 > 화학·나노과학전공 > Journal papers
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