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Shear stress tolerance of Streptococcus mutans aggregates determined by microfluidic funnel device (μFFD)

Title
Shear stress tolerance of Streptococcus mutans aggregates determined by microfluidic funnel device (μFFD)
Authors
Shumi W.Kim S.H.Lim J.Cho K.-S.Han H.Park S.
Ewha Authors
조경숙박성수
SCOPUS Author ID
조경숙scopus
Issue Date
2013
Journal Title
Journal of Microbiological Methods
ISSN
0167-7012JCR Link
Citation
vol. 93, no. 2, pp. 85 - 89
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Dental caries are initiated by the attachment of Streptococcus mutans aggregates to the surface of teeth. Bacterial adhesion to the interproximal space, the space between adjacent teeth, has not been investigated due to the lack of devices that mimic the space. Herein, we describe a method for determining the effect of shear stress and sucrose on the attachment of S. mutans aggregates to the interproximal space using microfluidic funnel device (μFFD). Using μFFD, the shear stress tolerance of sucrose-independent and sucrose-dependent S. mutans aggregates (larger than 50 μm in diameter) trapped in the funnel was tested against various flow rates of saliva solution (5 to 50 μl/min). Sucrose-independent aggregates were completely removed from the funnel walls at a low flow rate (10 μl/min) within 7. min., while sucrose-dependent aggregates were removed from the walls only at higher flow rates (25 and 50 μl/min) within several minutes. These results suggest that sucrose-dependent aggregates are more tolerant of shear stress than sucrose-independent aggregates, and are more likely to remain in the region with the smallest shear stress in the teeth. © 2013 Elsevier B.V.
DOI
10.1016/j.mimet.2013.02.004
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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