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dc.contributor.author이공주-
dc.contributor.author신동해-
dc.date.accessioned2016-08-28T10:08:42Z-
dc.date.available2016-08-28T10:08:42Z-
dc.date.issued2013-
dc.identifier.issn0907-4449-
dc.identifier.otherOAK-9920-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/223577-
dc.description.abstractNm23-H1/NDPK-A, a tumour metastasis suppressor, is a multifunctional housekeeping enzyme with nucleoside diphosphate kinase activity. Hexameric Nm23-H1 is required for suppression of tumour metastasis and it is dissociated into dimers under oxidative conditions. Here, the crystal structure of oxidized Nm23-H1 is presented. It reveals the formation of an intramolecular disulfide bond between Cys4 and Cys145 that triggers a large conformational change that destabilizes the hexameric state. The dependence of the dissociation dynamics on the H2O2 concentration was determined using hydrogen/deuterium-exchange experiments. The quaternary conformational change provides a suitable environment for the oxidation of Cys109 to sulfonic acid, as demonstrated by peptide sequencing using nanoUPLC-ESI-q-TOF tandem MS. From these and other data, it is proposed that the molecular and cellular functions of Nm23-H1 are regulated by a series of oxidative modifications coupled to its oligomeric states and that the modified cysteines are resolvable by NADPH-dependent reduction systems. These findings broaden the understanding of the complicated enzyme-regulatory mechanisms that operate under oxidative conditions. © 2013 International Union of Crystallography Printed in Singapore - all rights reserved.-
dc.languageEnglish-
dc.titleStructure of Nm23-H1 under oxidative conditions-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume69-
dc.relation.indexSCOPUS-
dc.relation.startpage669-
dc.relation.lastpage680-
dc.relation.journaltitleActa Crystallographica Section D: Biological Crystallography-
dc.identifier.doi10.1107/S0907444913001194-
dc.identifier.wosidWOS:000316743300020-
dc.identifier.scopusid2-s2.0-84875478027-
dc.author.googleKim M.-S.-
dc.author.googleJeong J.-
dc.author.googleShin D.-H.-
dc.author.googleLee K.-J.-
dc.contributor.scopusid이공주(7501497635)-
dc.contributor.scopusid신동해(15920079700)-
dc.date.modifydate20200221081000-
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약학대학 > 약학과 > Journal papers
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