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Comparison of RNA- and DNA-based bacterial communities in a lab-scale methane-degrading biocover

Title
Comparison of RNA- and DNA-based bacterial communities in a lab-scale methane-degrading biocover
Authors
Kim T.G.Moon K.-E.Yun J.Cho K.-S.
Ewha Authors
조경숙김태관
SCOPUS Author ID
조경숙scopus
Issue Date
2013
Journal Title
Applied Microbiology and Biotechnology
ISSN
0175-7598JCR Link
Citation
vol. 97, no. 7, pp. 3171 - 3181
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Methanotrophs must become established and active in a landfill biocover for successful methane oxidation. A lab-scale biocover with a soil mixture was operated for removal of methane and nonmethane volatile organic compounds, such as dimethyl sulfide (DMS), benzene (B), and toluene (T). The methane elimination capacity was 211 ± 40 g m-2 d-1 at inlet loads of 330-516 g m-2 d-1. DMS, B, and T were completely removed at the bottom layer (40-50 cm) with inlet loads of 221.6 ± 92.2, 99.6 ± 19.5, and 23.4 ± 4.9 mg m-2 d-1, respectively. The bacterial community was examined based on DNA and RNA using ribosomal tag pyrosequencing. Interestingly, methanotrophs comprised 80 % of the active community (RNA) while 29 % of the counterpart (DNA). Types I and II methanotrophs equally contributed to methane oxidation, and Methylobacter, Methylocaldum, and Methylocystis were dominant in both communities. The DNA vs. RNA comparison suggests that DNA-based analysis alone can lead to a significant underestimation of active members. © 2012 Springer-Verlag.
DOI
10.1007/s00253-012-4123-z
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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