View : 202 Download: 0
Distinct TLR-mediated pathways regulate house dust mite-induced allergic disease in the upper and lower airways
- Distinct TLR-mediated pathways regulate house dust mite-induced allergic disease in the upper and lower airways
- Ryu J.-H.; Yoo J.-Y.; Kim M.-J.; Hwang S.-G.; Ahn K.C.; Ryu J.-C.; Choi M.-K.; Joo J.H.; Kim C.-H.; Lee S.-N.; Lee W.-J.; Kim J.; Shin D.M.; Kweon M.-N.; Bae Y.S.; Yoon J.-H.
- Ewha Authors
- 배윤수; 김재상
- SCOPUS Author ID
- 배윤수; 김재상
- Issue Date
- Journal Title
- Journal of Allergy and Clinical Immunology
- Journal of Allergy and Clinical Immunology vol. 131, no. 2, pp. 549 - 561
- SCIE; SCOPUS
- Document Type
- Background: Allergic rhinitis (AR) and asthma are 2 entities of allergic airway diseases that frequently occur together, which is referred to as united airways. In contrast to this general concept, we hypothesized that innate immunity of the upper and lower airways is respectively distinctive, because the immunologic conditions of the nasal and lung mucosa as well as the functions of the immune cells within their epithelia are different. Objective: We wanted to identify distinctive mechanisms of innate immunity in the nose and lung mucosa, which are responsible for house dust mite (HDM)-induced AR and allergic asthma (AA), respectively. Methods: We constructed a mouse model of AR or AA induced by sensitization and consequent provocation with HDM extracts. Results: HDM-derived β-glucans, rather than LPS, were proven to be essential to activating innate immunity in the nasal mucosa and triggering AR, which depended on Toll-like receptor 2 (TLR2), but not on TLR4; however, the LPS/TLR4 signaling axis, rather than β-glucans/TLR2, was critical to HDM-induced AA. These differences were attributed to the specific role of β-glucans and LPS in inducing the surface expression of TLR2 and TLR4 and their translocation to lipid rafts in nasal and bronchial epithelial cells, respectively. We also showed that dual oxidase 2-generated reactive oxygen species mediate both β-glucan-induced TLR2 activation and LPS-induced TLR4 activation. Conclusions: We describe a novel finding of distinctive innate immunity of the nose and lungs, respectively, which trigger AR and AA, by showing the critical role of HDM-induced TLR activation via dual oxidase 2-mediated reactive oxygen species. © 2012 American Academy of Allergy, Asthma & Immunology.
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.