View : 30 Download: 0

Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities

Title
Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities
Authors
Kim T.G.Yi T.Cho K.-S.
Ewha Authors
조경숙김태관
SCOPUS Author ID
조경숙scopus
Issue Date
2013
Journal Title
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
ISSN
1093-4529JCR Link
Citation
vol. 48, no. 4, pp. 417 - 421
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Isolation of reference DNA templates for quantitative real-time PCR assays is an expensive, labor-intensive and time-consuming process if they are not readily available. Two artificial DNA templates with multiple probe sites were designed for quantifying methanogens and their 10 subgroups, based on the methyl coenzyme M reductase gene (mcrA). Their standards were comparable to each other. PCR amplification efficiencies (cycle vs. cumulative fluorescence) of the artificial DNAs were also comparable to those of the observed methanogen groups from anaerobic digesters. The artificial templates can be alternatives to the actual references. © 2013 Copyright Taylor and Francis Group, LLC.
DOI
10.1080/10934529.2013.728915
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE