Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이수영 | * |
dc.date.accessioned | 2016-08-28T10:08:18Z | - |
dc.date.available | 2016-08-28T10:08:18Z | - |
dc.date.issued | 2013 | * |
dc.identifier.issn | 1932-6203 | * |
dc.identifier.other | OAK-9651 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223342 | - |
dc.description.abstract | Although the role of glycogen synthase kinase 3β (GSK3β) in osteogenic differentiation of bone marrow-derived mesenchymal stromal cells (BMSCs) is well-characterized as a negative regulator of β-catenin, its effect on osteogenesis of adipose-derived stromal cells (ADSCs) is poorly understood. Here, we show that GSK3β positively regulates osteogenic differentiation of murine ADSCs. Gain-of-function studies showed that GSK3β promotes in vitro osteogenesis of ADSCs. Regulation of GSK3β activity in ADSCs, either by small interfering RNA (siRNA)-mediated GSK3β silencing or by pharmacological inhibitors, blunted osteogenesis and the expression of osteogenic markers. Importantly, we demonstrated that transgenic mice, engineered to overexpress the constitutively active GSK3β (GSK3β-S9A) mutant, exhibited a marked increase in osteogenesis, whereas expression of the catalytically inactive GSK3β (GSK3β-K85A) in mice inhibits osteogenic differentiation. Molecular analyses showed that the enhanced osteoblast differentiation induced by GSK3β was mediated by downregulation of β-catenin. Remarkably, β-catenin silencing enhances osteogenesis and osteoblast marker gene expression such as alkaline phosphatase (ALP) and osterix. Taken together, these findings demonstrate a novel role for GSK3β in the regulation of osteogenic differentiation in ADSCs. © 2013 Huh et al. | * |
dc.language | English | * |
dc.title | Glycogen Synthase Kinase 3β Promotes Osteogenic Differentiation of Murine Adipose-Derived Stromal Cells | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 8 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | PLoS ONE | * |
dc.identifier.doi | 10.1371/journal.pone.0054551 | * |
dc.identifier.wosid | WOS:000313682700125 | * |
dc.identifier.scopusid | 2-s2.0-84872480311 | * |
dc.author.google | Huh J.-E. | * |
dc.author.google | Ko R. | * |
dc.author.google | Jung H.J. | * |
dc.author.google | Lee S.Y. | * |
dc.contributor.scopusid | 이수영(53980218900;7409697278) | * |
dc.date.modifydate | 20240415140424 | * |