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Increase of organic solvent tolerance of Escherichia coli by the deletion of two regulator genes, fadR and marR

Title
Increase of organic solvent tolerance of Escherichia coli by the deletion of two regulator genes, fadR and marR
Authors
Oh H.Y.Lee J.O.Kim O.B.
Ewha Authors
김옥빈
SCOPUS Author ID
김옥빈scopus
Issue Date
2012
Journal Title
Applied Microbiology and Biotechnology
ISSN
0175-7598JCR Link
Citation
vol. 96, no. 6, pp. 1619 - 1627
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The improvement of bacterial tolerance to organic solvents is a main prerequisite for the microbial production of biofuels which are toxic to cells. For targeted genetic engineering of Escherichia coli to increase organic solvent tolerances (OSTs), we selected and investigated a total of 12 genes that participate in relevant mechanisms to tolerance. In a spot assay of 12 knockout mutants with n-hexane and cyclohexane, the genes fadR and marR were finally selected as the two key genes for engineering. Fatty acid degradation regulon (FadR) regulates the biosynthesis and degradation of fatty acids coordinately, and the multiple antibiotic resistance repressor (MarR) is the repressor of the global regulator MarA for multidrug resistance. In the competitive growth assay, the ΔmarR mutant became dominant when the pooled culture of 11 knockout mutants was cultivated successively in the presence of organic solvent. The increased OSTs in the ΔmarR and ΔfadR mutants were confirmed by a growth experiment and a viability test. The even more highly enhanced OSTs in the ΔfadR ΔmarR double mutant were shown compared with the two single mutants. Cellular fatty acid analysis showed that the high ratio of saturated fatty acids to unsaturated fatty acids plays a crucial role in OSTs. Furthermore, the intracellular accumulation of OST strains was significantly decreased compared with the wild-type strain. © Springer-Verlag Berlin Heidelberg 2012.
DOI
10.1007/s00253-012-4463-8
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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