View : 101 Download: 0

Tonsil-derived mesenchymal stromal cells: Evaluation of biologic, immunologic and genetic factors for successful banking

Title
Tonsil-derived mesenchymal stromal cells: Evaluation of biologic, immunologic and genetic factors for successful banking
Authors
Ryu K.-H.Cho K.-A.Park H.S.Kim J.-Y.Woo S.-Y.Jo I.Choi Y.H.Park Y.M.Jung S.-C.Chung S.M.Choi B.-O.Kim H.S.
Ewha Authors
정성민유경하최병옥우소연김한수정성철조인호최윤희박영미조경아박혜상
SCOPUS Author ID
정성민scopus; 유경하scopus; 우소연scopus; 김한수scopus; 정성철scopus; 조인호scopus; 최윤희scopus; 박영미scopus; 조경아scopus
Issue Date
2012
Journal Title
Cytotherapy
ISSN
1465-3249JCR Link
Citation
vol. 14, no. 10, pp. 1193 - 1202
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background aims. Although mesenchymal stromal cells (MSC) from human palatine tonsils (tonsillar MSC, T-MSC) have been isolated, whether T-MSC isolated from multiple donors are feasible for cell banking has not been studied. Methods. T-MSC before and after a standard protocol of cryopreservation and thawing were assessed regarding several basic characteristics, including colony-forming unitfibroblast features, MSC-specific surface antigen profiles, and inhibition of alloreactive T-cell proliferation. In vitro mesodermal differentiation potentials to adipocytes, osteocytes and chondrocytes were detected by staining with either cell-specific dyes or antibody after incubation with each appropriate differentiation medium. Expression of mesoderm-specific genes was also quantified by real-time polymerase chain reaction (PCR) assay. Expression profiles of endoderm-specific genes were identified by reverse transcription PCR assay. The feasibility of T-MSC in future engraftment was tested by short tandem repeat (STR) analysis using genomic DNA isolated randomly from three independent subjects. Results. Both fresh and cryopreservedthawed T-MSC showed a similar high proliferation capacity and expressed primitive cell-surface markers. Hematopoietic cell markers, HLA-DR, co-stimulatory molecules and follicular dendritic cell markers were not detected. In addition to mesodermal differentiation, fresh and cryopreservedthawed cells also underwent endodermal differentiation, as evidenced by the expression of endoderm-specific genes including forkhead box A2 (FoxA2), SIX homeobox 1 (Six1) and chemokine (C-C motif) ligand 21 (CCL21). Both cells significantly decreased phorbol 12- myristate 13-acetate (PMA)-induced T-cell proliferation. T-MSC from three independent donors formed chimerism in STR analysis. Conclusions. Our results demonstrate for the first time that T-MSC are a potentially good source for MSC banking. © 2012 Informa Healthcare.
DOI
10.3109/14653249.2012.706708
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE