Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정성민 | * |
dc.contributor.author | 유경하 | * |
dc.contributor.author | 최병옥 | * |
dc.contributor.author | 우소연 | * |
dc.contributor.author | 김한수 | * |
dc.contributor.author | 정성철 | * |
dc.contributor.author | 조인호 | * |
dc.contributor.author | 최윤희 | * |
dc.contributor.author | 박영미 | * |
dc.contributor.author | 조경아 | * |
dc.contributor.author | 박혜상 | * |
dc.date.accessioned | 2016-08-28T10:08:02Z | - |
dc.date.available | 2016-08-28T10:08:02Z | - |
dc.date.issued | 2012 | * |
dc.identifier.issn | 1465-3249 | * |
dc.identifier.other | OAK-9459 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/223181 | - |
dc.description.abstract | Background aims. Although mesenchymal stromal cells (MSC) from human palatine tonsils (tonsillar MSC, T-MSC) have been isolated, whether T-MSC isolated from multiple donors are feasible for cell banking has not been studied. Methods. T-MSC before and after a standard protocol of cryopreservation and thawing were assessed regarding several basic characteristics, including colony-forming unitfibroblast features, MSC-specific surface antigen profiles, and inhibition of alloreactive T-cell proliferation. In vitro mesodermal differentiation potentials to adipocytes, osteocytes and chondrocytes were detected by staining with either cell-specific dyes or antibody after incubation with each appropriate differentiation medium. Expression of mesoderm-specific genes was also quantified by real-time polymerase chain reaction (PCR) assay. Expression profiles of endoderm-specific genes were identified by reverse transcription PCR assay. The feasibility of T-MSC in future engraftment was tested by short tandem repeat (STR) analysis using genomic DNA isolated randomly from three independent subjects. Results. Both fresh and cryopreservedthawed T-MSC showed a similar high proliferation capacity and expressed primitive cell-surface markers. Hematopoietic cell markers, HLA-DR, co-stimulatory molecules and follicular dendritic cell markers were not detected. In addition to mesodermal differentiation, fresh and cryopreservedthawed cells also underwent endodermal differentiation, as evidenced by the expression of endoderm-specific genes including forkhead box A2 (FoxA2), SIX homeobox 1 (Six1) and chemokine (C-C motif) ligand 21 (CCL21). Both cells significantly decreased phorbol 12- myristate 13-acetate (PMA)-induced T-cell proliferation. T-MSC from three independent donors formed chimerism in STR analysis. Conclusions. Our results demonstrate for the first time that T-MSC are a potentially good source for MSC banking. © 2012 Informa Healthcare. | * |
dc.language | English | * |
dc.title | Tonsil-derived mesenchymal stromal cells: Evaluation of biologic, immunologic and genetic factors for successful banking | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 14 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1193 | * |
dc.relation.lastpage | 1202 | * |
dc.relation.journaltitle | Cytotherapy | * |
dc.identifier.doi | 10.3109/14653249.2012.706708 | * |
dc.identifier.wosid | WOS:000311182800006 | * |
dc.identifier.scopusid | 2-s2.0-84867657065 | * |
dc.author.google | Ryu K.-H. | * |
dc.author.google | Cho K.-A. | * |
dc.author.google | Park H.S. | * |
dc.author.google | Kim J.-Y. | * |
dc.author.google | Woo S.-Y. | * |
dc.author.google | Jo I. | * |
dc.author.google | Choi Y.H. | * |
dc.author.google | Park Y.M. | * |
dc.author.google | Jung S.-C. | * |
dc.author.google | Chung S.M. | * |
dc.author.google | Choi B.-O. | * |
dc.author.google | Kim H.S. | * |
dc.contributor.scopusid | 정성민(37042902400) | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.contributor.scopusid | 최병옥(7402755390) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.contributor.scopusid | 김한수(56509934900) | * |
dc.contributor.scopusid | 정성철(57008539100) | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.contributor.scopusid | 최윤희(7404776849) | * |
dc.contributor.scopusid | 박영미(7405372677) | * |
dc.contributor.scopusid | 조경아(21734204400) | * |
dc.date.modifydate | 20240422114059 | * |