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Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits
- Local BMP-7 release from a PLGA scaffolding-matrix for the repair of osteochondral defects in rabbits
- Jung M.R.; Shim I.K.; Chung H.J.; Lee H.R.; Park Y.J.; Lee M.C.; Yang Y.I.; Do S.H.; Lee S.J.
- Ewha Authors
- 이승진; 심인경
- SCOPUS Author ID
- Issue Date
- Journal Title
- Journal of Controlled Release
- Journal of Controlled Release vol. 162, no. 3, pp. 485 - 491
- SCI; SCIE; SCOPUS
- Document Type
- The use of tissue engineering to repair large osteochondral defects has been impeded by the limited regenerative capacity of cartilage. Herein, we describe the local release of bone morphogenetic protein 7 (BMP-7) to stimulate the bone marrow-derived progenitors to repair osteochondral defects. BMP-7-releasing poly(d,l-lactide-co-glycolide) (PLGA) matrix was specially designed to retain the dual-function of local BMP-7 release and progenitor-scaffolding with its defect-fitting architecture. To optimize the release kinetics during the repair period, BMP-7/PLGA film was cast on the surface of a cylindrical PLGA matrix. The matrix demonstrated a release profile of BMP-7 in a sustained manner over 28 days, maintaining its biological activity. The cylindrical PLGA matrices loaded with BMP-7 were implanted into the osteochondral defects (2 mm in diameter, 3 mm in depth) in rabbit knees. Histological observations revealed that neo-cartilage generation was completed in a well-integrated morphology with its surrounding normal cartilage and subchondral bone at 12 weeks post-implantation. Partial degradation of the PLGA matrix during the repair period guided neo-cartilage formation, which verified the effective scaffolding function of the matrix. Regenerated cartilage in BMP-7-treated defects stained positive for type II collagen and glycosaminoglycan (GAG). Adjacent BMP-7-untreated defects were also repaired with cartilage regeneration, suggesting the effect of local BMP-7 release in the synovial fluid. The BMP-7-unloaded PLGA matrix demonstrated guided cartilage regeneration to a certain extent, whereas the adjacent defects without the matrix revealed only fibrous tissue infiltration. These results indicated that a strategy employing the combined functions of local BMP-7 release and the cell scaffolding of a PLGA matrix might be a potential modality for osteochondral repair. © 2012 Elsevier B.V. All rights reserved.
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