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Characterization of curli a production on living bacterial surfaces by scanning probe microscopy

Title
Characterization of curli a production on living bacterial surfaces by scanning probe microscopy
Authors
Oh Y.J.Cui Y.Kim H.Li Y.Hinterdorfer P.Park S.
Ewha Authors
박성수
Issue Date
2012
Journal Title
Biophysical Journal
ISSN
0006-3495JCR Link
Citation
vol. 103, no. 8, pp. 1666 - 1671
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Curli are adhesive surface fibers produced by many Enterobacteriaceae, such as Escherichia coli and Salmonella enterica. They are implicated in bacterial attachment and invasion to epithelial cells. In this study, atomic force microscopy was used to determine the effects of curli on topology and mechanical properties of live E. coli cells. Young's moduli of both curli-deficient and curli-overproducing mutants were significantly lower than that of their wild-type (WT) strain, while decay lengths of the former strains were higher than that of the latter strain. Surprisingly, topological images showed that, unlike the WT and curli-overproducing mutant, the curli-deficient mutant produced a large number of flagella-like fibers, which may explain why the strain had a lower Young's modulus than the WT. These results suggest that the mechanical properties of bacterial surfaces are greatly affected by the presence of filamentous structures such as curli and flagella. © 2012 Biophysical Society.
DOI
10.1016/j.bpj.2012.09.004
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자연과학대학 > 화학·나노과학전공 > Journal papers
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