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Peroxiredoxin III and sulfiredoxin together protect mice from pyrazole-induced oxidative liver injury

Title
Peroxiredoxin III and sulfiredoxin together protect mice from pyrazole-induced oxidative liver injury
Authors
Bae S.H.Sung S.H.Lee H.E.Kang H.T.Lee S.K.Oh S.Y.Woo H.A.Kil I.S.Rhee S.G.
Ewha Authors
이서구배수한길인섭우현애
SCOPUS Author ID
이서구scopusscopus; 우현애scopus
Issue Date
2012
Journal Title
Antioxidants and Redox Signaling
ISSN
1523-0864JCR Link
Citation
vol. 17, no. 10, pp. 1351 - 1361
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Aims: To define the mechanisms underlying pyrazole-induced oxidative stress and the protective role of peroxiredoxins (Prxs) and sulfiredoxin (Srx) against such stress. Results: Pyrazole increased Srx expression in the liver of mice in a nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent manner and induced Srx translocation from the cytosol to the endoplasmic reticulum (ER) and mitochondria. Pyrazole also induced the expression of CYP2E1, a primary reactive oxygen species (ROS) source for ethanol-induced liver injury, in ER and mitochondria. However, increased CYP2E1 levels only partially accounted for the pyrazole-mediated induction of Srx, prompting the investigation of CYP2E1-independent ROS generation downstream of pyrazole. Indeed, pyrazole increased ER stress, which is known to elevate mitochondrial ROS. In addition, pyrazole upregulated CYP2E1 to a greater extent in mitochondria than in ER. Accordingly, among Prxs I to IV, PrxIII, which is localized to mitochondria, was preferentially hyperoxidized in the liver of pyrazole-treated mice. Pyrazoleinduced oxidative damage to the liver was greater in PrxIII -/- mice than in wild-type mice. Such damage was also increased in Srx -/- mice treated with pyrazole, underscoring the role of Srx as the guardian of PrxIII. Innovation: The roles of Prxs, Srx, and ER stress have not been previously studied in relation to pyrazole toxicity. Conclusion: The concerted action of PrxIII and Srx is important for protection against pyrazole-induced oxidative stress arising from the convergent induction of CYP2E1-derived and ER stress-derived ROS in mitochondria. © 2012 Mary Ann Liebert, Inc.
DOI
10.1089/ars.2011.4334
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일반대학원 > 생명·약학부 > Journal papers
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