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Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC-MS/MS

Title
Quantitative determination of aceclofenac and its three major metabolites in rat plasma by HPLC-MS/MS
Authors
Kim E.Ahn B.Noh K.Kang W.Gwak H.S.
Ewha Authors
곽혜선
SCOPUS Author ID
곽혜선scopus
Issue Date
2012
Journal Title
Journal of Separation Science
ISSN
1615-9306JCR Link
Citation
vol. 35, no. 17, pp. 2219 - 2222
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We developed a method for the simultaneous quantification of aceclofenac and its three major metabolites in rat plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard (IS), aceclofenac, diclofenac, 4'-hydroxyaceclofenac, 4'-hydroxydiclofenac, and the IS were chromatographed on a reverse-phase C18 analytical column. The isocratic mobile phase of acetonitrile/0.1% formic acid (aq; 9:1 [v/v]) was eluted at 0.3 mL/min. Quantification was performed on a triple-quadrupole mass spectrometer using electrospray ionization, and the ion transitions were monitored in selective reaction-monitoring mode. The coefficient of variation in the assay precision was less than 8%, and the accuracy was 92-103%. This method was successfully used to measure the concentrations of aceclofenac and its three major metabolites in rat plasma following the oral administration of a single 20 mg/kg oral dose of aceclofenac. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
DOI
10.1002/jssc.201200367
Appears in Collections:
약학대학 > 약학과 > Journal papers
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