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Effective Layer by Layer Cell Seeding into Non-Woven 3D Electrospun Scaffolds of Poly-L-Lactic Acid Microfibers for Uniform Tissue Formation

Title
Effective Layer by Layer Cell Seeding into Non-Woven 3D Electrospun Scaffolds of Poly-L-Lactic Acid Microfibers for Uniform Tissue Formation
Authors
Kang J.K.Lee M.H.Kwon B.J.Kim H.H.Shim I.K.Jung M.R.Lee S.J.Park J.-C.
Ewha Authors
이승진심인경
SCOPUS Author ID
이승진scopus
Issue Date
2012
Journal Title
Macromolecular Research
ISSN
1598-5032JCR Link
Citation
vol. 20, no. 8, pp. 795 - 799
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
A major challenge in the use of electrospun scaffolds for tissue generation is that tightly packed layers of nanofibers can block cell infiltration into the scaffold, resulting in non-homogeneous cell distribution. This can cause incomplete extracellular matrix deposition and failure to generate tissue. In this study, we developed a cell seeding method that supports cell infiltration and distribution within a three-dimensional (3D) flexible and shape-controllable electropun scaffold. Cells were seeded on the scaffold layer-by-layer and cell growth rate 7 days post seeding was compared to cell growth after traditional cell seeding. Scanning electron microscopy demonstrated that the shape-controlled scaffold consist of microfibers with uniform thickness. In using the layer-by-layer seeding method, we observed that the cells were distributed uniformly throughout the 3D scaffold. In contrast, use of the traditional cell seeding method resulted in the cells remaining on the surface of the scaffold. These results suggest that a layer-by-layer cell seeding method can improve cell distribution within a 3D scaffold and effectively facilitate tissue formation.
DOI
10.1007/s13233-012-0117-z
Appears in Collections:
약학대학 > 약학과 > Journal papers
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