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IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells

Title
IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells
Authors
Kim B.-S.Kim H.-J.Kim J.S.You Y.-O.Zadeh H.Shin H.-I.Lee S.-J.Park Y.-J.Takata T.Pi S.-H.Lee J.You H.-K.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2012
Journal Title
Bone
ISSN
8756-3282JCR Link
Citation
vol. 51, no. 3, pp. 506 - 514
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The exact molecular mechanisms governing the differentiation of bone marrow stromal stem/progenitor cells (BMSCs) into osteoblasts remain largely unknown. In this study, a highly expressed protein that had a high degree of homology with interferon-induced transmembrane protein 1 (IFITM1) was identified using differentially expressed gene (DEG) screening. We sought to determine whether IFITM1 influenced osteoblast differentiation. During differentiation, IFITM1 expression gradually increased from 5 to 10. days and subsequently decreased at 15. days in culture. Analysis of IFITM1 protein expression in several cell lines as well as in situ studies on human tissues revealed its selective expression in bone cells and human bone. Proliferation of human alveolar-derived bone marrow stromal cells (hAD-BMSCs) was significantly inhibited by IFITM1 knockdown by using short hairpin RNA, as were bone specific markers such as alkaline phosphatase, collagen type I α 1, bone sialoprotein, osteocalcin, and osterix were decreased. Calcium accumulation also decreased following IFITM1 knockdown. Moreover, IFITM1 knockdown in hAD-BMSCs was associated with inhibition of Runx2 mRNA and protein expression. Collectively, the present data provide evidence for the role of IFITM1 in osteoblast differentiation. The exact mechanisms of IFITM1's involvement in osteoblast differentiation are still under investigation. © 2012 Elsevier Inc.
DOI
10.1016/j.bone.2012.05.012
Appears in Collections:
약학대학 > 약학과 > Journal papers
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