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Reactive oxygen species mediated DNA damage is essential for abnormal erythropoiesis in peroxiredoxin II -/- mice

Title
Reactive oxygen species mediated DNA damage is essential for abnormal erythropoiesis in peroxiredoxin II -/- mice
Authors
Kwon T.-H.Han Y.-H.Hong S.G.Lee D.J.Ha H.-L.Kang S.W.Li W.Yoon D.Y.Yu D.-Y.
Ewha Authors
강상원이두재
SCOPUS Author ID
강상원scopus; 이두재scopus
Issue Date
2012
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 424, no. 1, pp. 189 - 195
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Erythroid cells are highly prone to oxidative damage generated during erythropoiesis and thus are well equipped with antioxidant defense systems. However, their roles have been poorly characterized. Here, we investigated the role of peroxiredoxin II in mouse erythropoiesis. Loss of Prx II significantly increased apoptosis and cell cycle arrest leading to abnormal erythropoiesis at 3weeks of age when erythropoietin levels were almost same between wild type and Prx II -/-. In Prx II -/- bone marrow cells, DNA tail length as an indicator of the oxidative damage was greatly increased and mRNAs of the molecules associated with DNA damage and repair and transcription regulators of antioxidant enzymes were also significantly increased. In addition, N-Acetyl-l-Cysteine treatment significantly decreased immature erythroblasts and apoptotic cells increased in Prx II -/- BMCs. These results strongly demonstrate that Prx II plays an essential role in maintaining normal erythropoiesis by protecting DNA damage. © 2012 Elsevier Inc.
DOI
10.1016/j.bbrc.2012.06.113
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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