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Fluorescence ratiometric zinc sensors based on controlled energy transfer

Title
Fluorescence ratiometric zinc sensors based on controlled energy transfer
Authors
Woo H.You Y.Kim T.Jhon G.-J.Nam W.
Ewha Authors
전길자남원우유영민
SCOPUS Author ID
전길자scopus; 남원우scopus; 유영민scopus
Issue Date
2012
Journal Title
Journal of Materials Chemistry
ISSN
0959-9428JCR Link
Citation
vol. 22, no. 33, pp. 17100 - 17112
Indexed
SCOPUS WOS scopus
Abstract
The high-fidelity detection of labile zinc is of central importance for understanding the molecular mechanisms that link zinc homeostasis and human pathophysiology. Fluorescence ratiometric sensors are most suitable for the detection and trafficking of intracellular zinc ions. Here, we report the development of fluorescence ratiometric zinc sensors (HN1 and HN2) based on two-fluorophore platforms. The sensor constructs include blue fluorescent umbelliferone and an energy-accepting chromophore that absorbs the blue fluorescence. Zinc binding was found to promote fluorescence turn-on of the umbelliferone emission by suppression of intramolecular photoinduced electron transfer, thereby facilitating resonance energy transfer to the energy acceptors. The net observables were the fluorescence ratiometric changes, the extent of which depended strongly on the chemical structures of the acceptors. Photophysical investigations, including steady-state and transient photoluminescence spectroscopy, suggested a mechanism for the fluorescent zinc response that involved a combination of the intramolecular electron transfer and the interchromophoric energy transfer. The zinc probes displayed sensing capability that is suitable for the detection of biological zinc ions, with good selectivity, pH tolerance, and appropriate K d values. Finally, zinc detection was demonstrated by fluorescence ratiometric visualization of exogenously supplied zinc ions in live HeLa cells. The probes enabled the reliable monitoring of zinc equilibration across the cell membrane. © The Royal Society of Chemistry 2012.
DOI
10.1039/c2jm33366j
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자연과학대학 > 화학·나노과학전공 > Journal papers
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