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Bioprocess engineering to produce 10-hydroxystearic acid from oleic acid by recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia

Title
Bioprocess engineering to produce 10-hydroxystearic acid from oleic acid by recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia
Authors
Jeon E.-Y.Lee J.-H.Yang K.-M.Joo Y.-C.Oh D.-K.Park J.-B.
Ewha Authors
박진병양경미
SCOPUS Author ID
박진병scopus
Issue Date
2012
Journal Title
Process Biochemistry
ISSN
1359-5113JCR Link
Citation
vol. 47, no. 6, pp. 941 - 947
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Microbial hydroxylation of long chain fatty acids has been extensively investigated. However, biotransformation productivity remains below ca. 1.0 g/g cell dry weight (CDW)/h under process conditions. In the present study, a highly efficient microbial hydroxylation process to convert oleic acid into 10-hydroxystearic acid was developed. A recombinant Escherichia coli expressing ohyA, the gene encoding oleate hydratase of Stenotrophomonas maltophilia, was used as the biocatalyst. Investigation of the ohyA expression and biotransformation conditions (e.g., inducer concentration, gene expression period before initiating biotransformation, mixing condition of reaction medium) enabled 10-hydroxystearic acid to accumulate to a final concentration of approximately 46 g/L in the culture medium. The specific product formation rate and product yield reached approximately 2.0 g/g CDW/h (i.e., 110 U/g CDW) and 91%, respectively. The specific product formation rate was more than 3-fold higher than those of a bioprocess using wild type Stenotrophomonas sp. cells. Additionally, the product of the whole-cell biotransformation was recovered at a yield of 70.9% and a purity of 99.7% via solvent fraction crystallization at low temperature. These results will contribute to developing a biological process for hydroxylation of oleic acid. © 2012 Elsevier Ltd.
DOI
10.1016/j.procbio.2012.03.002
Appears in Collections:
엘텍공과대학 > 식품공학전공 > Journal papers
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