View : 52 Download: 0

Cellular response to poly(vinyl alcohol) nanofibers coated with biocompatible proteins and polysaccharides

Title
Cellular response to poly(vinyl alcohol) nanofibers coated with biocompatible proteins and polysaccharides
Authors
Lee S.Y.Jang D.H.Kang Y.O.Kim O.B.Jeong L.Kang H.K.Lee S.J.Lee C.-H.Park W.H.Min B.-M.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2012
Journal Title
Applied Surface Science
ISSN
0169-4332JCR Link
Citation
vol. 258, no. 18, pp. 6914 - 6922
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A PVA nanofibrous matrix was prepared by electrospinning an aqueous 10 wt% PVA solution. The mean diameter of the PVA nanofibers electrospun from the aqueous PVA solution was 240 nm. The water resistance of the as-spun PVA nanofibrous matrix was improved by physically crosslinking the PVA nanofibers by heat treatment at 150 °C for 10 min. In addition, the heat-treated PVA nanofibrous matrix was coated with biocompatible polysaccharides (chitosan (CHI) or hyaluronic acid (HA)) and proteins (collagen (COL) or silk fibroin (SF)) to construct biomimetic nanofibrous scaffolds. The coating of proteins or polysaccharides on the PVA nanofibrous matrix was confirmed by ATR-IR spectra, and the degree of coating was determined by elemental analysis based on nitrogen content. The coated PVA matrices exhibited less hydrophilicity, except for the HA coating, and better tensile properties than the pure PVA nanofibrous matrix. The increase in tensile properties was due to interfiber bonds formed by the coating. The effect of protein and polysaccharide coating on normal human keratinocytes (NHEKs) and fibroblasts (NHEFs) was examined by cytocompatibility assessment in vitro. Among the CHI-, COL-, HA- and SF-coated PVA matrices, the SF-coated PVA nanofibrous matrix was found to be the most promising scaffold for the attachment and spreading of NHEKs and NHEFs as compared to the pure PVA matrix. This approach to controlling the surface properties of nanofibrous structures with SF may be useful in the design and tailoring of novel matrices for skin regeneration. © 2012 Elsevier B.V.
DOI
10.1016/j.apsusc.2012.03.135
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE