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The isolation and in situ identification of MSCs residing in loose connective tissues using a niche-preserving organ culture system

Title
The isolation and in situ identification of MSCs residing in loose connective tissues using a niche-preserving organ culture system
Authors
Choi M.-Y.Kim H.-I.Yang Y.-I.Kim J.T.Jang S.H.Park C.M.Jang W.H.Youn Y.-C.Cheong S.-H.Choi C.S.Kim D.K.Lee S.J.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2012
Journal Title
Biomaterials
ISSN
0142-9612JCR Link
Citation
vol. 33, no. 18, pp. 4469 - 4479
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Mesenchymal stem cells (MSCs) have been discovered in a multitude of organs, but their distribution and identity are still uncertain Furthermore, loose connective tissue (LCT) is dispersed throughout virtually all organs, but its biological role in tissue homeostasis is unclear Here, we describe a unique organ culture system to explore the omnipresence and in situ identity of MSCs among the LCTs This culture system included the use of the fibrin hydrogel coupled with dynamic culture conditions, using native LCTs obtained from various organs as starting materials This culture allowed MSC outgrowth into the hydrogel to be robustly supported, while maintaining the structural integrity of LCTs during in vitro culture Subcultured outgrown cells fulfilled the minimal requirements for defining MSCs on the basis of clonogenicity, multipotency, and immunophenotypic characteristics In vitro label-retaining assay demonstrated that the numbers of mobilized and proliferated cells in situ increased in the pericapillary region and expressed both MSCs and pericytes markers, indicating that the in situ identity of MSCs represents a certain population of pericapillary pericytes Our results indicate that this culture system affords a unique strategy for both isolating MSCs and recapitulating their niche in LCTs © 2012 Elsevier Ltd.
DOI
10.1016/j.biomaterials.2012.03.009
Appears in Collections:
약학대학 > 약학과 > Journal papers
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