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Effect of pH on the iron autoxidation induced DNA cleavage

Title
Effect of pH on the iron autoxidation induced DNA cleavage
Authors
Kim J.-M.Oh B.N.Kim J.-H.Kim S.K.
Ewha Authors
김진흥
SCOPUS Author ID
김진흥scopus
Issue Date
2012
Journal Title
Bulletin of the Korean Chemical Society
ISSN
0253-2964JCR Link
Citation
vol. 33, no. 4, pp. 1290 - 1296
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Fenton reaction and iron autoxidation have been debated for the major process in ROS mediated DNA cleavage. We compared both processes on iron oxidation, DNA cleavage, and cyclic voltammetric experiment at different pHs. Both oxidation reactions were preferred at basic pH condition, unlike DNA cleavage. This indicates that iron oxidation and the following steps probably occur separately. The ROS generated from autoxidation seems to be superoxide radical since sod exerted the best inhibition on DNA cleavage when H 2O 2 was absent. In comparison of cyclic voltammograms of Fe 2+ in NaCl solution and phosphate buffer, DNA addition to phosphate buffer induced significant change in the redox cycle of iron, indicating that iron may bind DNA as a complex with phosphate. Different pulse voltammogram in the presence of ctDNA suggest that iron ions are recyclable at acidic pH, whereas they may form an electrically stable complex with DNA at high pH condition.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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