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LIME mediates immunological synapse formation through activation of VAV

Title
LIME mediates immunological synapse formation through activation of VAV
Authors
Son M.Park I.Lee O.-H.Rhee I.Park C.Yun Y.
Ewha Authors
윤영대박인영
SCOPUS Author ID
윤영대scopus; 박인영scopus
Issue Date
2012
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 33, no. 4, pp. 407 - 414
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Lck Interacting Membrane protein (LIME) was previously characterized as a transmembrane adaptor protein mediating TCR-dependent T cell activation. Here, we show that LIME associates with Vav in response to TCR stimulation and is required for Vav guanine nucleotide exchange factor (GEF) activity for Rac1. Consistent with this finding, actin polymerization at the immunological synapse (IS) was markedly enhanced by overexpression of LIME, but was reduced by expression of a LIME shRNA. Moreover, TCR-mediated cell adhesion to ICAM-1, laminin, or fibro-nectin was downregulated by expression of LIME shRNA. In addition, in the IS, LIME but not LAT was found to localize at the peripheral-supramolecular activation cluster (p-SMAC) where the integrins were previously shown to be localized. Together, these results establish LIME as a transmembrane adaptor protein linking TCR stimulation to IS formation and integrin activation through activation of Vav. © 2012 KSMCB.
DOI
10.1007/s10059-012-0011-8
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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