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Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

Title
Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome
Authors
Lee Y.J.Choi I.-K.Sheen Y.Y.Park S.N.Kwon H.J.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2012
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 33, no. 3, pp. 309 - 316
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens. © 2012 KSMCB.
DOI
10.1007/s10059-012-2280-7
Appears in Collections:
약학대학 > 약학과 > Journal papers
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