View : 19 Download: 0

Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma

Title
Moesin is a biomarker for the assessment of genotoxic carcinogens in mouse lymphoma
Authors
Lee Y.J.Choi I.-K.Sheen Y.Y.Park S.N.Kwon H.J.
Ewha Authors
신윤용
SCOPUS Author ID
신윤용scopus
Issue Date
2012
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 33, no. 2, pp. 203 - 210
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
1,2-Dibromoethane and glycidol are well known genotoxic carcinogens, which have been widely used in industry. To identify a specific biomarker for these carcinogens in cells, the cellular proteome of L5178Y mouse lymphoma cells treated with these compounds was analyzed by 2-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry (MS). Of 50 protein spots showing a greater than 1.5-fold increase or decrease in intensity compared to control cells on a 2-D gel, we focused on the candidate biomarker moesin. Western analysis using monoclonal rabbit anti-moesin confirmed the identity of the protein and its increased level of expression upon exposure to the carcinogenic compounds. Moesin expression also increased in cells treated with six additional genotoxic carcinogens, verifying that moesin could serve as a biomarker to monitor phenotypic change upon exposure to genotoxic carcinogens in L5178Y mouse lymphoma cells. © 2012 KSMCB.
DOI
10.1007/s10059-012-2271-8
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE