View : 19 Download: 0

Determination of a novel TAZ modulator, 2-butyl-5-methyl-6-(pyridine-3-yl)-3-[2'-(1H-tetrazole-5-yl)-biphenyl-4-ylmethyl]-3H imidazo[4,5-b]pyridine] (TM-25659) in rat plasma by liquid chromatography-tandem mass spectrometry

Title
Determination of a novel TAZ modulator, 2-butyl-5-methyl-6-(pyridine-3-yl)-3-[2'-(1H-tetrazole-5-yl)-biphenyl-4-ylmethyl]-3H imidazo[4,5-b]pyridine] (TM-25659) in rat plasma by liquid chromatography-tandem mass spectrometry
Authors
Choi S.H.Lee K.-R.Woo J.-C.Kim N.J.Moon D.C.Hwang E.S.Ahn S.-H.Bae M.A.Kim M.-S.
Ewha Authors
황은숙
SCOPUS Author ID
황은숙scopus
Issue Date
2012
Journal Title
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085JCR Link
Citation
vol. 63, pp. 47 - 52
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
TM-25659 compound, a novel TAZ modulator, is developed for the control of bone loss and obesity. TAZ is known to bind to a variety of transcription factors to control cell differentiation and organ development. A selective and sensitive method was developed for the determination of TM-25659 concentrations in rat plasma. The drug was measured by liquid chromatography-tandem mass spectrometry after liquid-liquid extraction with ethyl acetate. TM-25659 and the internal standard imipramine were separated on a Hypersil GOLD C18 column with a mixture of acetonitrile-ammonium formate (10. mM) (90:10, v/v) as the mobile phase. The ions m/z 501.2 → 207.2 for TM-25659 and m/z 281.0 → 86.0 for imipramine in multiple reaction monitoring mode were used for the quantitation. The calibration range was 0.1-100μg/ml with a correlation coefficient greater than 0.99. The lower limit of quantitation of TM-25659 in rat plasma was 0.1μg/ml. The percent recoveries of TM-25659 and imipramine were 98.6% and 95.7% from rat plasma, respectively. The intra- and inter-batch precisions were 3.17-15.95% and the relative error was 0.38-10.82%. The developed assay was successfully applied to a pharmacokinetic study of TM-25659 administered intravenously (10. mg/kg) to rats. © 2012 Elsevier B.V..
DOI
10.1016/j.jpba.2012.01.036
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE