View : 12 Download: 0

A pyrene-imidazolium derivative that selectively Recognizes G-Quadruplex DNA

Title
A pyrene-imidazolium derivative that selectively Recognizes G-Quadruplex DNA
Authors
Kim H.N.Lee E.-H.Xu Z.Kim H.-E.Lee H.-S.Lee J.-H.Yoon J.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2012
Journal Title
Biomaterials
ISSN
0142-9612JCR Link
Citation
vol. 33, no. 7, pp. 2282 - 2288
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
G-quadruplexes, formed of four stranded guanine bases stabilized by monovalent cations, serve important role in cancer cell growth and control gene expression in telomere. Since there are various types of quadruplex structures, rapid and simple screening methods with high selectivity, sensitivity and nontoxicity are required for understanding about the biological roles of quadruplex DNA as well as in designing therapeutics. Herein, we report a pyrene-imidazolium derivative, JY-1, which can with high selectivity recognize G-quadruplex using fluorescence and NMR spectroscopy. This is the first example based on the imidazolium derivative, which can detect the G-quadruplex directly to utilize the excimer/monomer emission change in pyrene fluorophore. The selectivity of strong binding to a specific sequence can allow for quadruplex sensing and the detection method presented here is very simple, using fluorescence and NMR study. Also, the groove binding characteristic of JY-1 to the G-quadruplex has a relatively low nonspecific toxicity and the structure-specific differences in fluorescent character between DNA duplex and G-quadruplex may offer more discovery and application in biological study. © 2011 Elsevier Ltd.
DOI
10.1016/j.biomaterials.2011.11.073
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE