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Determination of daumone in mouse plasma by HPLC/MS-MS

Title
Determination of daumone in mouse plasma by HPLC/MS-MS
Authors
Noh K.Park J.H.Kim M.Jung M.Lee H.J.Kwon K.-I.Kang W.Ha H.
Ewha Authors
이화정하헌주
SCOPUS Author ID
이화정scopus; 하헌주scopus
Issue Date
2012
Journal Title
Biomedical Chromatography
ISSN
0269-3879JCR Link
Citation
vol. 26, no. 2, pp. 152 - 155
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Daumone, a pheromone secreted by Caenorhabditis elegans, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS-MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5-week repeated oral administration of daumone in mice. © 2011 John Wiley & Sons, Ltd.
DOI
10.1002/bmc.1649
Appears in Collections:
약학대학 > 약학과 > Journal papers
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