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Assessing effects of earthworm cast on methanotrophic community in a soil biocover by concurrent use of microarray and quantitative real-time PCR

Title
Assessing effects of earthworm cast on methanotrophic community in a soil biocover by concurrent use of microarray and quantitative real-time PCR
Authors
Kim T.G.Moon K.-E.Lee E.-H.Choi S.-A.Cho K.-S.
Ewha Authors
조경숙김태관이은희
SCOPUS Author ID
조경숙scopus; 이은희scopusscopus
Issue Date
2011
Journal Title
Applied Soil Ecology
ISSN
0929-1393JCR Link
Citation
vol. 50, no. 1, pp. 52 - 55
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Effects of earthworm cast (EC) on the methanotrophic community in a soil biocover were evaluated using microarray and quantitative real-time PCR (qRT-PCR). Soil was collected from a biocover with either soil alone or a mixture of soil and EC (3:1, w/w). The microarray results showed a more diverse methanotrophic community in the EC biocover than that in the soil biocover (p< 0.05). A principal component analysis result confirmed a substantial change in the methanotrophic community structure due to the added EC. Type I methanotrophs dominated both biocovers, with Methylobacter being most abundant. The qRT-PCR results showed that EC greatly increased the methanotrophic population levels (p< 0.05) up to 100 fold. In conclusion, EC can increase the population density of methanotrophs as well as their diversity, resulting in a substantial shift in the community structure. The results confirmed the promising potential of EC as a bed material for enhancing the biocover performance. © 2011 Elsevier B.V.
DOI
10.1016/j.apsoil.2011.07.011
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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